Quantification of Steroids and 25-OH Vitamin D3 in Dried Blood Spots and Serum using LC/MS/MS

Steroids are important biomarkers for a number of clinical conditions. Most clinical assays for steroid quantification are based on immunoassays, which suffer from selectivity problems due to cross-reactivity, or GC/MS separations, which are time consuming and often involve complex sample preparation procedures. Analysis of a comprehensive steroid panel is challenging due to the need to rapidly separate structurally related compounds in order to quantify low concentrations present in complex biological specimens. LC/MS/MS has been introduced as an alternative for steroid analysis in the past few years due to its superior sensitivity and selectivity.